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PROJECTS OF 2006-08 BATCH of M.Sc (BIOTECHNOLOGY)



    

Sl. No.

Name

% of marks (upto 2nd sem)

Title of project

Name of the Institute

Name of the Guide

1

Chandana Roy

73.0

“Analysis of genetic variation in different banana variety(Musa species)using Random Amplified  Polymorphic DNA”.

 

Brief : This work is an attempt which has been made to fingerprint & study their genetic relationship, genetic variability & phylogeny using RAPD marker,which could be useful for germplasm management , crop imporovement, detection of gene flow between species.

CRRI, Cuttack.

Dr.S.C.Sahu (Principal Scientist)

2

Sunita Sahoo

70.5

“Estimation of malaria incidence at the community level and comparision of positivity of P.Faccparum with Haemoglobin% in the peripheral, ICTKIT and Cord/placental blood of pregnant lady”.

 

Brief :  The work describes the endemicity of malaria in a particular forest fringed region and the transmission of malaria from pregnant women to child is very high and in pregnant lady we can detect the malaria parasite only by doing PCR of cord and placental blood.

RMRC (Regional Medical Research Centre).

Dr.A.Mohapatra, (Scientist, RMRC)

3

Satyajita Mishra

68.6

“Assessment of genetic diversity in “lablab”species through molecular markers”.

 

 Brief :  The project describes the genetic characterization of 14 varieties of lablab species was done using two PCR – based markers namely RAPD and ISSR. Ten decamer oligoncleatide primers were tested out of which six primer produced reproducible amplifications . Similarly out of 5ISSR primers 3 primers gave reproducible results. A total of 149 bands were amplified with these primers. Acladograms was derived for the RAPD and ISSR pull data from jaccard’s similarly coefficient following UPGMA method using Shan clustering. All variety sharing a common node of 30 %.

ITER, Pharmaceutical College (Center of Biotechnology, Siksha’O’Anusandhan University, Bhubaneswar)

Dr.Laxmikanta Acharya

4

Saumyaparna Chakraborty

67.6

“Construction of promoter-reporter gene fusions to monitor inductionof virulence genes of vibrio cholerae.”

 

Brief : Vibrio Cholera, a noninuasive enteric bacteria, is the causative agent of the dairrhael disease cholera. The production of cholera toxin(CT) & toxin co-regulated pilus(TCP) are the major virulence factors, ToxR,ToxT & ToxS are the transcriptional regulator required for the expression of the ctx-gene encoding CT by different environmental factors like temperature & pH, the ctx promoter was fused to a reporter lact gene, the construct was introduced into the V.cholerae chromosomes & lact expression was studied at different temperature & pH.

Department of Infectious Diseases in IICB, Kolkata.

Dr.Ruksana Chowdhury (Scientist E)

5

Nisha Jha

67.0

“Comparative study on hematological & solid tumors by cytogenetics & molecular genetics approach.”

 

Brief : Out of number of cancers, fresh blood samples of AML and lung cancer were collected and analysed for karyotyping and genotyping from karyotyping analysis it was found that chromosome 15 in lung cancer was found to be frequently abberated from PCR and PCR based RFLP of AML heterozygous Mutant(arg/pro)was obtained and of lung cancer heterozygous Mutant (arg/pro), Homozygous Mutant (arg/arg) was observed.

The R & D phase of IACI & RC

Dr.Mrs.Kaiser Jamil (Research Director, IACI & RC)

6

Soumesh kumar Padhi

67.0

“Prevalance of anaemia in children by estimation of Hb and ferritin.”

 

Brief :  The main objective is detection of concentration of Hb and ferritin in the blood samples by Drabkkaing’s and ELISA method respectively and effect of low concentration of Hb and ferritin on the children causing Anaemia.

RMRC, Bhubaneswar

Dr.Gandham Bulliyye. ( Director of Human Genetics at RMRC)

7

Sumana Tribedi

66.5

“Polymerase Chain Reaction (PCR) based detection of Diarrhaeagenic E.coli.”

 

Brief :   This study was performed with the objective of isolation of E.coli from stool specimes, Pathogrouping of diarrhaeagenic E.coli by virulence genes targeted multiplex PCR, and seratyping of isolates, harbouring the virulence genes, with the positive isolates further studies can be performed specially on antimicrobial resistance, plasmid analysis etc.

National Institute of Cholera & Enteric Diseases(NICED, Kolkata)

Dr.T.Ramamurthy, (Deputy Director of NICED)

8

Nishi Bhamra

66.0

“DNA fingerprinting on varieties of rice using RAPD markers to evaluate the genetic diversity present in rice varieties such as Ramakrishna, Salkati, Pooja, IR64, TN1, Swarna.”

CRRI, Cuttack.

Dr.S.C.Sahu (Principal Scientist)

9

Mousumi Biswas

65.8

“Expression of few immune related genes in Indian major carp, “Labio rohita” in response to “Aeromonas hydrophila” infection.

 

Brief : Labio rohita, the major Indian carp was challenged with infective Aeromonas Hydrophila and tissue samples were collected after various specific time intervals. RNA extraction was done from these tisssues under aseptic conditions. cDNA was prepared through the process of reverse transcription (RT) and then annealed with different primers for the expression was qualified for each of the gene expressed.

Central Institute of Freshwater Aquaculture

Dr.P.K.Sahoo, Senior Scientist, Dept of Pathology.

10

Leepika kumari Das

64.3

“Analysis of microbial load in cow milk at different storage conditions.”

 

Brief:  Cow milk collected from 25 cows in & around Bhubaneswar was screened for bacterial isolates. All the 25 samples were divided into 5 groups & stored in different conditions like at room temp for 8 hrs (group I), at 4 degree c/8hrs (groupie), 24 hrs at room temp(group III), at a degree for 24 hrs (group IV) & rest 5 samples were preserved on such at room temp for 24 hrs & microbial load was estimated.

Department of Bacteriology & Virology, Orissa Veterinary College, OUAT, Bhubaneswa

Dr.Rajashree Mishra. (Asst.Prof)

Microbiology

11

Shalina Behera

63.8

“Production & characterization of xylanase from fungi isolated from waterhyacinth plant (Eicchornia crassipes).”

 

Brief : This work describes the production of xylanse by the unknown fungal strain and optimization of the conditions suitable for xylanase production by that strain. In addition characterization of enzyme are also investigated. The study will assist further in determining the suitability of the enzyme for industrial application.

North East Institute of Science & Technology, Jorhat, Assam

Dr.M.Khongsai, (Scientist)

12

Suman kumari Singh

62.6

“To find out variations in transthyretin gene in Indian Population and its association with Spinocerebellar ataxia”

 

Brief : The project describes that the amybidosis are diseases of secondary proteins structure in which a normally soluble protein  forms insoluble extracellular fibril deposits causing organ dysfunction. One of the 20 proteins that form human amyloid fibril are thransthyretin(TTR). Ataxia is a neurological disorder Amylodosis cause polyneuropathy and ataxia also cause polyneurapathy. So on this basis we are trying to find out if there is any association between TTR mutations and spinocerebellar Ataxia.

Department of Molecular Biology, IGIB, Delhi

Dr.Abhay Sharma (Scientist)

13

Aasita Apoorva

62.1

“Agrobacterium rhizogenes – medicated Genetic Transformation of Mentha arvensis L.and  Gymnema sylvestre R.Br and Hairy Root Culture of Gymnema sylvestre R.Br”

Birla Institute of Technology,Mesra, Ranchi

Dr.Ramesh Chandra. (Sr.Lecturer)

14

Preetam Samant

62.0

“Analysis of microbial load in Buffalo milk at different storage condition.”

 

Brief : Buffalo milk collected from 25 buffaloes in & around Bhubaneswar were screened for bacterial isolates. All the 25 samples were divided into 5 groups & stored in different conditions like at room temp for 8 hrs (group I), at 4 degree c/8hrs (groupie), 24 hrs at room temp(group III), at a degree for 24 hrs (group IV) & rest 5 samples were preserved on such at room temp for 24 hrs & microbial load was estimated.

Department of Bacteriology & Virology, Orissa Veterinary College, OUAT, Bhubaneswar.

Dr.Rajashree Mishra. (Asst.Prof)

Microbiology

15

Sisir kumar Dash

61.0

“Genetic relationship & diversity between Mangifera species revealed by RAPD analysis”

 

Brief : Through this project the genetic instability, variation in chromosome, % of mutation gene flow inside the gene pool can be determined  by making linkage map & sequencing. By knowing the gene sequence the better hybrid of plant species can be produced. The transformation in the genome of the plant can be done after knowing the linkage mapping and sequencing.

CRRI, Cuttack

Dr.S.C.Sahu, (Principal Scientist)

16

Manini Samal

60.5

“Callusing and regeneration on Stevia Plant”

Central Rice Research Institute(CRRI)

Dr.Sarat ch Sahoo (Principal)

17

Jyoti Prasad Lahan

59.0

“Study on antifungal activity of plant extracts (solanum melongena) against Human pathogenic fungi causing superficial skin diseases.”

 

Brief :  During the project investigation plant materials by using different solvents  hane been extracted and tested in vitro efficacy of plant extracts against human pathogenic fungi. Among the various extracts chlomoform extract is more effective.

Defence Research Laboratory, Tezpur Assam.

Jayashree Das (HOD, Biotechnology Division)

18

Leena Parida

56.3

“Maleria during Pregnancy – PCR positivity in comparison to peripheral and ICT kit with Hb% of cord and placental Blood & estimation of malaria incidence at community level.”

 

Brief : Malaria is a major cause of morbility and mortality in tropical countries like South African countries, India etc. Orissa contributes 225 of all malaria cases, 43% of all cases of plasmodium falciparum malaria and 50% of all malaria attributable deaths reported in the country. So in this project we compare the PCR positivity in comparison to peripheral, ICT kit with Hb% of cord and placental blood and also estimated the malaria incidence at the community level in the Niladri block of Khurda district.

Regional Medical Research Centre (ICMR)

Dr.Amarendra Mohapatra (Asst Director)

19

Deepta Bhanoo Somaprov

56.0

“Intenspecific variation of Genomic Characteristics of Gasteria revealed through RAPD markers.

 

Brief :  The project starts with isolation of the sample, isolation of DNA, Qualification of DNA concentration, PCR amplification using random primers & finally the electrophonesis & visualization step of the amplified products.

Regional Plant Resource Centre Lab.

Mr.A.B.Das (Senior Scientist)

20

Rubi Kumari

54.5

“Isolation and characterization of Rhizobium isolated from Leguminous Lac Hostplauts”

 

 

 

Brief :  the project describes that Rhizobium helps in nitrogen fixation, found in the root nodules of leguminous plants. Rhizobium helps in increasing the production of leguminous plants. Since Rhizobium is of great importance from both agricultural and atmospheric point of view. So it provides a wide range of area in its research and development area.

IINRG, Namkum.

Dr.D.N.Saha (Senior Scientist)

21

Parbin Kausar

53.8

“Analysis of microbial load in Goat milk at different storage conditions.”

 

Brief : Goat  milk collected from 25 goats in & around Bhubaneswar were screened for bacterial isolates. All the 25 samples were divided into 5 groups & stored in different conditions like at room temp for 8 hrs (group I), at 4 degree c/8hrs (groupie), 24 hrs at room temp(group III), at a degree for 24 hrs (group IV) & rest 5 samples were preserved on such at room temp for 24 hrs & microbial load was estimated.

Department of Bacteriology & Virology, Orissa Veterinary College, OUAT, Bhubaneswa

Dr.Rajashree Mishra. (Asst.Prof)

Microbiology

22

Tushar Kanti Saha

53.8

“A Comparative study of the Immune Status of Malignant Brain Tumor following treatment like Immunotherapy.”

Cellular & Molecular Immunology Lab, Institute of Post Graduate Medical Education & Research.

Dr.Swapna Chaudhuri, (Lecturer, Dept. of Physiology, IPMER, Kolkata)

23

Bijay Buragohain

53.3

“To evaluate the antidermatophytic activity of medicinal plant(Ocimum Sanctum)”.

 

Brief :  During the project work, the extracted  plant material using solvents like petroleum elher, chloroform, methnol etc and then tested the invitro efficacy of the plant extracts, petroleum elher was found to be more effective against the tested dermatophytes.

Biotechnology Division of Defence Research Lab, Tezpur, Assam

Mrs.Jayashree Das (Scientist)

 

 

 

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