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PROJECTS OF 2005-07 BATCH of M.Sc (BIOTECHNOLOGY)
 

Sl No

Name

MSc Marks %

Title of Project

Name of Institutions

1

CHINMAYEE MOHAPATRA(W)

76.3

Title : Marker assisted selection & SSR of bacterial blight resistance gene(Xa21,Xa13 & Xa5).  

Brief : Inthis study, both parents i.e.Lalat(susceptible parent) and IRBB 60(gene pyramid of IR 24 carrying resistance gene xa-5,xa-13 and xa-21) and the newly developed gene pyramids in the background of Lalat(carrying different combination of resistance genes) were analyzed by PCR with Sequence Tagged Site(STS) markers RG556, RG136 and pTA248 for xa-5,xa-13 and xa-21 respectively.

CRRI, Cuttack.

 

Guide: G.J.N.Rao

 

2

ANWESHA CHATTERJEE(W)

75.0

Title : PCR based molecular diagnosis of Leprosy(M. leprae). 

Brief :   The project describes the PCR analysis of tissues for M.leprae DNA provides a valuable means for identifying this organism. Mutation in M.Laprae genome those are associated with resistance to different drugs used against this pathogen have been identified. So it is regarded as the PCR is the better diagnostic tool for the detection of leprosy at an early stage. The primer Ri & Rn are very specific for M.Laprae & were absent in 20 other mycobacterail species.

Dr.B.C.Ray Institute of Postgraduate &
Medicinal Research, Kolkata.

 Guide :  Dr.Basudev Bhattacharya.
(Senior Faculty, Deptt of Biochemistry &
Biophysics)

3

ANURADHA KUMARI(W)

74.4

Corelation of MIU, MBV by Serology through advance chmiluminescence with real time PCR. 

C.M.R.I( B.M.Birla) Kolkata.

4

CHRISTINA PRIYANKA SEKAR(W)

72.1

Title : Phytochemical investigation of  Litsea  glulinosa & evaluation of its  biological activity. 

Brief : The project aims at finding the never therapentic agents from herbs, the phylachemical and antiinflamatory studies of the plant, litsea glutinose linn(lauraceae) has been taken for investigation. The bark was extracted  and the % yield, colour and the consistency of the extract was observed. The extract was analysed for the presence of various groups of phyloconstituents.The study showed the presence of carbohydrates, proteins, tannins, saponins, steroids & alkaloids.

Utkal University,
Dept. of Pharmaceutical Sciences, BBSR.

 

Guide :  Dr.Sagar Kumar Mishra

5

SWAGATIKA SAHU(W)

71.3

Title : Effect if inorganic & bioorganic fertilizers on the growth of hybrid papaya  under pot culture condition .

Rrief : The project describes organic fertilizers along with fungi were applied on plants growing in greenhouse. It was found that the mixed treatment of organic fertilizers and fungi showed good morphotical characters like survivility, shoot, root ratio, stem biomass etc. Different organic fertilizers were used for experiment.

R.P.R.C.,BBSR

 

Guide : Dr.(Mrs)Nibha Gupta    (Sr.Scientist) 

 

6

SHAHILA PARWEEN(W)

70.9

Title : Pharmacoproteomics: study of the effect of herbal drugs in the cancer of Male Wistar Rats. 

Brief : The project shows rat models challenged for monitoring the hepatoprotective action of natural products which shows considerable results. SGPT, SGOT are also increased in Silymarin & Extract treated group. Sudden decrease in serum enzymes after challenging with hepatotoxins(AAF,CCI4) in extract treated group. Total protein content was seen to decrease when assayed at different time interval.

Jamia Hamdard,

New Delhi.

7

ANIKET RAKSHIT

70.6

Effects of plant product as biopesticides  on  Sitophilus oryzae.

Indian Statistical Institute,Kolkata. 

8

SMRUTI DAS(W)

70.6

Title : Biochemical characterization of wild variety of Rice. 

Brief : The project describes Rice is one of the staple food in Asia where it provides 40% - 70% of food calories. The quality of the seed is a major determination of the qquantity of the o/p of the corp. Riceseed yield are detrimentally affected by lodging results in difficult and prolonged harvesting. The poorly filled gain may have high gain moisture content, lower specific weight and it may even have sprouted and be discoloured due to pathogenic activity. The objectivity of the study is to characterize the changes in biochemical, seed p pattern of storage proteins in seed of wild species of rice under controlled and accelerated aging condition.

C.R.R.I., Cuttack.

 

Guide : Dr.Prameela Krishnan. (Sr Scientist)

9

JAYDEEP CHAUDHURI

70.2

Gene expression during host pathogen interaction. 

Indian Institute of Chemical Biology.

10

SWARANIKA PODDAR(W)

69.0

Title : Nanosilica drug against silk worm efficacy & molecular mechanism of action.

Brief : The project describes that in the sericulture industry, BmNPV, which is a pathogen of the mulberry silkwormBombyn mori, is of great economic concern. Till now there is no permanent presentive method for the occurrence and the spr the effect of nanosilica drug on silkworm larvae which was infected by Bombyn mori nucleopolyhedrosis virus(BmNPV)

Indian Statistical Institute,Kolkata

 

Guide : Dr.Arunava Guswami.
Head Nanobiotechnology Lab

11

SAUMYA BANDYOPADHYAY(W)

68.9

Title : Construction of cDNA libraries: generation of ESTs for heat stressed variety of Pigeon pea. 

Brief : Pigeon & the young leaves were frozen immediately at liquid N2 for total RNA extraction using Trizol method. mRNA is present in very less quantity in the total RNA cDNA was also prepared from the extracted mRNA by reverse transcription & amplified by PCR.

I.A.R.I., New Delhi.

 

Guide : Dr.R.C.Bhattacharya.

 

12

SUJAYA NAYAK(W)

68.5

Characterization & isolation of microbes present in 30 ton heap 

Institute of mineral &
material technology (IIMT),BBSR.

13

KAUSAR E RAHAMAN

68.3

Title : Industrial microbiology & quality control.

Brief : The project shows that yeast culture in large scale in large fermenter requires various analysis like quality of molasses, determination of fermentation capacity of yeast strain growth rate etc. The molasses used for fermentation was characterized by various test like test for dry matter, total reducing sugar, fermentable sugar, nitrogen phosphorus content etc. All these test of the molassed quality will ultimately effect the yeast production. 

Kalyani yeast, Kalyani.

 

 

Guide  : Mrinal Chakraborty. Manager – Q.C

14

SUJATA DASH(W)

68.3

Title : Effect of bioinoculants on the growth of hybrid Drum stick (PKM-I) & its Rhizospheric microflora under greenhouse condition. 

Brief : The project describes the effect of Bioinoculants on the growth of hybrid drumstick(PKM1) & its Rhizaspheric Microflora under green house condition was studied. Analysis of growth parameters such as no. of leaves, root length, % of germination, no of leaflets, ratio of root & shoot length were done by taking averages, standard deviation & test of significance experimentation of bioinoculants on Hybrid Drumstick has been found that applications of microbial sources are beneficial as compared to chemical fertilizers for the plants.

R.P.R.C.,BBSR.

 

 

Guide ; Dr.Nibha Gupta. Sr.Scientist. 

 

 

15

LOVALIN BHOI(W)

67.5

Molecular characterization & finger printing of Breast cancer gene by AP-PCR. 

Sangene Biotech PVT,LTD

16

RUCHIRA SAHA(W)

67.5

Title : Real Time PCR & its applications. 

Brief :  The project describes the Traditional PCR is measured at the end points(plateau). While use of real time PCR in collecting data in the exponential growth phase. In this case the cleared probe provides a permanent record amplification of an amplicon. No post PCR processing due to closed system i.e.no electrophoretical separation of amplified DNA.

B.M.B.H.R.C., Kolkata.

Guide : Dr.Indranil Roy 

 

 

17

ASHUTOSH GUPTA

67.4

 Title : Identification of Hepatitis C  Virus in blood sample from HCL injected & HIV co injected Patients.  

Brief :  In this project HCV is known to have marked genetic heterogenecity and it was estimated to have a nucleotide substitution rate between 1.44 x 10-3 & 1.92 x 10-3 substitution site per year. Accumulation of nucleotide substitution in HCV genome results in the diversification & evolution of the different genotypes. There is increasing evidence that patients injected with different HCV genetype may have different clinical profiles, severity of liver disease & response to interferon alfa therapy.

In this study the positive samples found out by ELISA are again screened by Tridot rapid test. The RNA was isolated & amplified. The genotyping was done following the Ohno T method in which 2 different mixtures of primers are used for specific genetyping.

I.C.M.R Virus Unit Kolkata.

 

Guide :  Dr.Provash Chandra
Sadhukhan. (Research Officer)

 

 

 

 

 

18

BIJOYA DATTA  CHOWDHURY(W)

67.3

Title : Effect of nanoparticles on Tilapia  species & studied on their molecular behaviour. 

Breif :  The project shows that Aquaculture  particularly inland fishing is a vital source of protein & income. Tilapia Fish(Oreochromics mossanlices & O niloticus) is of great economic importance in fishing industry & is highly commercial. It is dir=urnal & mainly feeds on phytoplanktons & alga. Such conditions can be created in vitro to observe the effect of surface modified nanoparticles. In the present study the beneficial effect of surface modified nanosilicas have been done & also to see the effects on the growth of tilapia fishes.

Indian Statistical Institute,Kolkata.

 

Guide: Dr.Arrunava Goswami.
Head Nanobiotechnology Lab.

 

19

URAKSHI(W)

67.2

Studies on optimization of regeneration & transformation of Brassica oleracea  with PBECK 2000.3 & RAPD analysis.

Jamia Hamdard,

New Delhi.

20

UTPAL MANDAL

67.0

Antitumour  promoting activity of Diphenyl methyl selenocyrate

C.NC.I., Kolkata.

21

REKHA YADAV(W)

66.9

Isolation & characterization of PSB from LD stage.

R.R.L., BBSR.

22

KAUSTAV CHOWDHURY

66.7

Title : Agroeutamotoxic nanoparticle used to control stored grain pest tested on Sitophillus oryzae. 

Brief : The project shows development of nanosilica soil to be used as pesticides. These pesticides would not leave toxic residues or contaminate the soil. The mode of action of these nanosilica to be used as pesticide has been studied. The secondary products of plant like alkaloids,pyrithrins which are insecticidual in nature along with nanasilica can be potential pesticides without detrimental effect on environment.

Indian Statistical Institute,Kolkata. 

23

SONALI PATTNAIK(W)

66.2

To identify phylogenetic relationship among Nymphea,Nelumbo,Euryale using RAPD & ISSR marker. 

R.P.R.C.,BBSR

24

RUPESH KUMAR HOTA

65.8

Title : Zymographic detection & clinical correlation of MMP in the plasma/sera of cervical cancer patient.

Brief : The project describes that Cervical cancer is a malignancy of cervix and it stands as the second most common cancer in terms of both medicine & mortality. Elevated expression of MMp-2(Gelatinase A) & MMP-9(Gelatinase B) was seen in human cancer, including cervical carcinoma. MMP – 2 & MMP – 9 are inactive proenzymes that undergo activation  followed by cleanage of an NH-2 terminal prodomain. MMPs are considered as the key enzyme that plays a key role in tumor cell invasion & metastasis

Sangene biotech Pvt. Ltd.,

 

Guide : Shins.K Jose  

 

 

 

25

SUBHASH LAHIRI

65.3

Title : Temporal expression of E.R in the development of  Malphigian tubules of D. melanogaster. 

Brief : The project describes Analogous organ of vertebrate renal tubule in the prosophila in malphigian tubules which perform osmoregularory roles. Complex regulatory pathways are associated with the multiple cell types present in tubules. The metamorphosis pathway of the insect is carried out by steroid hormone ecdysone/ECR/USP complex activates downstream processes signal pathway. In this work the specific requirements is Malphigian tubules.

Cytogenetic Lab Dept. of Zoology, B.H.U

 

Guide : Dr.(Mrs) Madhu Gwaldas Tapadia. 

 

 

26

APURBA CHHOTARAY(W)

65.1

Title : Detection  of Aflatoxin +ve  strains & quatification of  aflatoxin from Aspergillus flavus strains. 

Brief : The project shows that Aspergillus flavus was cultured on B-CD-PDA medium that produced aflatoxin. The isolates produced yellow pigment which turned pink when ammonia solutions was added to it. Addition of an excess of any acid converts black colour to yellow indicating that the pigments which form the basis for this test, thereby acting as a PH indicator dye. Isolates were classified as low, intermediate & high producer of aflatoxin on the basis of intensity of pink color produced by them.

C.R.R.I, Cuttack.

 

Guide : Dr.S.K.Mohanty. Principal Scientist

 

 

 

27

AMBARISH PRASAD DAS

65

Study of genetic diversity of Machilus bombycina with molecular marker RAPD. 

R.R.L Jorhat.

28

SMITA BISI(W)

65.0

Title : Study on development of Zinc tolerant plant N.tabaccum. 

Brief : This project is for better understanding of adaptation of metal tolerant plant, propagation of zinc tolerant plants can be useful for mine reclamation. This study helps to understand the basic mechanism of metal uptake. The plants were grown on MS medium supplemented with BAP & zinc.

R.P.R.C., BBSR.

 

Guide :  Dr.S.K.Palai. (Asst.Floriculturist) 

 

29

IPSITA MOHANTY(W)

64.5

Analysing the genetic diversity of the family ‘Annonaceae’ using molecular marker. 

R.P.R.C,BBSR

30

SHAISTA  JAHAN(W)

64.4

Title : Correlation of MTB in microbiological technique & real time PCR in Molecular Biology. 

Brief : The project describes Mycobacteruim Tuberculosis is the etiologic agent of tuberculosis in human whereas M.boris is the etiologic agent of TB in cows & rarely in humans. Humans can also be infected by the consumption of un-pasturized milk. The bacterium was detected by ZNstaining.

C.M.R.I., Kolkata.

 

Guide: Dr.Indrani Roy.

31

JYOTIRMAYEE SWAIN(W)

63.9

Title : Microbial analysis of post methylated distillery effluent & wet land treatment.

Brief : The project aims at the physicochemical parameters decrease manifolds after sequential treatment with bacteria and wetland plants. This was studied by analyzing the Distillery Effluent in Sequential Treatment with Bacteria and Wetland plants. The reason of the decrease being the digestion of complex compounds and heavy metals in the root nodules of the wetland plants, which contains Rhizispheric Bacteria in them. Thus, the uptake of these compounds reduces the Physico Chemical parameters to a great extent.   

I.T.R.C., Lucknow

Dr.Ram Chandra. 

 

 

 

 

32

PRIYANATH MUKHOPADHYAY

63.9

Title : Effect of Dopamine on IGFI induced growth of stomach cancer growth line(AGS).

Brief : The project describes the human stomach tumor cells contain a number of different growth factor receptors through which the tumor cells can exert considerable influence on the growth kinetics of stomach tumor.Dopamine, a catecholamine neutransmitter, is present in considerable amount in thenormal human stomachtissues. This dopamine controls different physiological functions of stomach including protection of gastric epithelical tissues from ulcer induced injury. The present study shows the inhibition of physiological concentration of dopamine on proliferation of human gastric cell line AGS. 

C.N.C.I

 

Guide : Dr.Partha Sarathi DasGupta
(Sr. Scientific Officer)

33

DEBASMITA DIKSHIT(W)

63.7

Title : Shoot regenararation  from the auxillary bud of phyllanthus amarunthus callus induction from leaf.

Brief :  The project shows the Micropropagation of medicinal herb Phyllanthusamarus was done using nodal segments. Induction of callus from leaves of P.amarus was chosen as the experimental material because of its multiple medicinal use. It also has hepatoprotective properties. Fresh roots are said to be beneficial in jaundice. The later is applied to offensive sores & ulcers.

I.M.M.T, BBSR

 

Guide : Dr.M.Thirunavoukkarasu. (Scientist)